Detection of Glu-Glu-Tagged Proteins in Mammalian Cell Culture Media by Dot Immunoblotting
نویسندگان
چکیده
منابع مشابه
Prev Glu Phe
Download curonidation is an important pathway in the metabolism of nicotine, with previous studies suggesting 22% of urinary nicotine metabolites are in the form of glucuronidated compounds. Recent in vitro studve suggested that the UDP-glucuronosyltransferases (UGT) 2B10 and 2B17 play major roles in nicotine onidation with polymorphisms in both enzymes shown to significantly alter the levels o...
متن کاملPCR-based markers for identification of some allelic variation at Glu-1 and Glu-3 loci in common wheat
Marker assisted selection (MAS) is a tool for breeding, screening, and genetic characterization of germplasm. Allelic variation of both high and low molecular weight glutenin subunits (HMW/LMW-GS) is associated with the rheological properties of wheat flour. In this study, we investigated glutenin pattern using SDS-PAGE and their PCR based on DNA markers in 60 advanced wheat lines and cultivars...
متن کاملCharacterization of apolipoprotein E7 (Glu
Previously, a mutant apolipoprotein (apo) E, apolipoprotein E7 (Glu 244 ➝ Lys, Glu 245 ➝ Lys), has been identified in association with hyperlipidemia and atherosclerosis. To investigate the effects of its structural changes on lipoprotein metabolisms and its correlation with atherosclerosis, we characterized this mutant apoE with respect to its receptor-binding, heparin-binding, and lipoprotein...
متن کاملPeptide-Based Optical uPAR Imaging for Surgery: In Vivo Testing of ICG-Glu-Glu-AE105
Near infrared intra-operative optical imaging is an emerging technique with clear implications for improved cancer surgery by enabling a more distinct delineation of the tumor margins during resection. This modality has the potential to increase the number of patients having a curative radical tumor resection. In the present study, a new uPAR-targeted fluorescent probe was developed and the in ...
متن کاملFunctional roles of Glu-269 and Glu-325 within the lactose permease of Escherichia coli.
Acidic residues which are found on transmembrane segments within the lactose permease may play an important role in H+ and/or sugar recognition. To examine the functional roles of Glu-269 and Glu-325, we have constructed a variety of amino acid substitutions (e.g. aspartate, glycine, alanine, serine, or glutamine) via site-directed mutagenesis. At position 269, all mutations appear to have a de...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BioTechniques
سال: 2000
ISSN: 0736-6205,1940-9818
DOI: 10.2144/00285st07